Table 2 of Varma, Mol Vis 2010; 16:501-505.

Table 2. Effect of caffeine on lens dulcitol.


Group		 Dulcitol
(µmoles/g wet wt.)
Galactose 17.2±2.5
Galactose + Caffeine 19.6±6

Rats weighing 40–45 g were fed a 24% galactose diet without or with 1% caffeine. There were then euthanized, lenses dissected out and as described, transferred on a Millipore grid and photographed by trans-illumination using 50 W daylight tube. Subsequently they were used to prepare a protein free filtrate by homogenization with Nelson-Semoygi reagent and centrifugation. Dulcitol was determined as described in the text. Values are expressed as mean±s.d., n=6 (number of animals).

Varma, Mol Vis 2010; 16:501-505. http://www.molvis.org/molvis/v16/a56

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