Table 2 of Asomugha, Mol Vis 2010; 16:476-494.

Table 2. Summary of crystallin species in spots from Coomassie-stained 2D-DIGE gel of 69-year-old human lens identified by ESI-QTRAP LC-MS/MS.

Spot number Identification by Q-TRAP Mr (kDa) Localization
1 γD <20 N>C
2 αA chain, αB <20 N<C
3 αA chain, αB, βA3, βB3 <20 N<C
4 αB, βA3, βB1, γD <20 N=C
5 αB, βA3, γD <20 N>C
6 αA, αB, βA3, γD, γS <20 N=C
7 αA, αB,βA3, βB1, βB2, γB, γS <20 N=C
8 αB, βA3 <20 N=C
8B αB, βA3 <20 N>C
9 αB, βA3, βA4 <20 N<C
10 αA,, αB, βA3, βA4 <20 N<C
11 αA, βA3, βA4 <20 N>C
11B αA,, αB, βA4 <20 N<C
12 αA, γS 20 - 35 N<C
12B αA, αB, βA3, βB1, βB2, γC, γS 20 - 35 N<C
13 αA, βA4, βB1, βB2, γS 20 - 35 N<C
14 βA3, βB1, γS 20 - 35 N=C
14B βB2 20 - 35 N<C
15 βA3, βB1, βB2, βA4, γS 20 - 35 N=C
15B βA3, βA4, βB2, γS 20 - 35 N<C
16 αA, βB1, βB2 20 - 35 N<C
17 truncated βB1 chain, βB1, βB2 20 - 35 N=C
18 βB1, βB2 20 - 35 N>C
19 αA chain, βB1, βB2 20 - 35 N>C
19B αA, αB, βA3, βB2, βB4 20 - 35 N<C
20 αB, βA3, βB1, βB2, γS 20 - 35 N=C
21 αA, αB, βA3, βB1, βB2 20 - 35 N<C
22 αA 20 - 35 N<C
23 NI >35 N>C
24 NI >35 N>C
25 NI >35 N>C
26 NI >35 N<C
27 NI >35 N>C
28 CP49 >35 N>C
29 NI >35 N>C
30 NI >35 N>C
31 NI >35 N>C
32 NI >35 N>C
33 NI >35 N>C
34 NI >35 N>C
35 αA, Filensin >35 N>C
36 NI >35 N>C

NI=Not Identified by mass spectrometry. Data from the “Localization” column was based on DIGE analysis.

Asomugha, Mol Vis 2010; 16:476-494. http://www.molvis.org/molvis/v16/a54

©2010 Molecular Vision http://www.molvis.org/molvis/

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