Figure 5. Standard 2D gel of a 69-year-old human lens. IEF was done in the first dimension using an 11 cm IPG strip, pH 5–8, followed by SDS–PAGE using a 15% polyacrylamide gel in the second dimension. The gel was stained with Coomassie blue R250, and shows a profile similar to 2D-DIGE gels of the same tissue. However, HMW (>35 kDa) aggregates are not distinguished as individual spots, rather they appeared as non-descript bands.