Figure 1. Tissue section of a human lens. A: Whole tissue section (12 μm) of 69-year-old lens stained with hematoxylin and eosin, as seen under a stereomicroscope with 4× magnification. All three major regions are present in this section, however the nucleus stained more faintly because of possible increased hydrophobicity of the proteins in this region. B: Unstained section showing three major lenticular regions: Nucleus (N), Inner Cortex (IC), and Outer Cortex (OC), as seen during LCM with the Zeiss/PALM Microbeam microscope at its lowest magnification of 2.5×.