Figure 8. Occurrence of high molecular weight proteins in lenticular urea-soluble protein fractions in different groups of Wistar rats. Detection of changes in high molecular weight insoluble protein by western blot analysis. Panels A, D, and G show immunoreactive bands of spectrin (240 kDa), vimentin (57 kDa), and α-actin (42 kDa), respectively. Box-plots show the band intensity of spectrin, vimentin, and α-actin, relative to the level in 10 day-old rat lenses (panels B, E, and H, respectively). Linear regression analysis revealed a significant negative correlation between the urea-soluble to soluble protein ratio and the levels of spectrin, vimentin, and alpha-actin (panels C, F, and I, respectively). (▲)­­ Values in 10 day-old rat lenses; (□) Values in 16 day-old rat lenses; (●) Values in 30 day-old rat lenses; (♦) Values in 90 day-old rat lenses; (×) Values in 180 day-old rat lenses; (■) Values in 365 day-old rat lenses; (○) Values in the lenses of 16 day-old rats that had been administered selenite on post-partum day 10.