Figure 7. Occurrence of high molecular weight protein in lenticular soluble protein fractions in different groups of Wistar rats. Detection of changes in high molecular weight soluble protein by western blot analysis. An equal amount of the soluble protein fractions were loaded in each lane and subjected to 4%–20% SDS–PAGE. 10 = 10 day-old rat lens and the standard M = Molecular weight marker (A). Blots were subjected to immunoblot detection. Immunoblot analysis did not reveal any differences in the fraction for high molecular weight proteins, vimentin (B) and α-actin (C), in all the groups measured.