Figure 5. Occurrence of βB1-crystallin in
lenticular soluble protein fractions in different groups of Wistar
rats. A typical western blot showing age-dependent relative
concentrations of βB1-crystallin in the soluble protein fractions of
Wistar rat lenses. A: An equal amount of protein was loaded in
each lane and subjected to electrophoresis. Blots were incubated with
βB1-crystallin antibody. B: band intensity of βB1-crystallin
levels relative to 10 day-old rat lenses. C: Linear regression
analysis revealed a significant negative correlation between
βB1-crystallin and the urea-soluble to soluble protein ratio. (▲)
Values in 10 day-old rat lenses; (□) Values in 16 day-old rat lenses;
(●) Values in 30 day-old rat lenses; (♦) Values in 90 day-old rat
lenses; (×) Values in 180 day-old rat lenses; (■) Values in 365 day-old
rat lenses; (○) Values in the lenses of 16 day-old rats that had been
administered selenite on post-partum day 10.