Figure 6. The Wnt inhibitor SFRP2 regulates cancer stem-like cells. A: The Wnt inhibitor SFRP2 was decreased in Weri and Y79 cells by addition of LiCl, as measured by QPCR on unsorted cells. The difference between 20 mM and 40 mM LiCl was statistically significant at p<0.05 (*) and the difference between treated and untreated was statistically significant at p<0.01 (**). The experiment was repeated four times. B: Reducing SFRP2 levels using siRNA (average % reduction was 63% relative to scrambled siRNA) increased stem cell marker gene expression. The increase in Oct3/4 expression was statistically significant at p=0.05 (*). The experiment was repeated four times.