Table 1 of Xu, Mol Vis 2010; 16:438-444. Table 1 of Xu, Mol Vis 2010; 16:438-444.
Table 1. Primers for sub-cloning of βA3 genes and site-directed mutagenesis.
| Constructs | Forward primer | Reverse primer |
|---|---|---|
| Sub-cloning | ||
| βA3-crystallin | TTGGATCCGAGACCCAGGCTGAG | GCTAAGCTTCTACTGTTGGATTCG |
| Mutagenesis | ||
| βA3ΔG91 | GTTTATCCTGGAGAGAGAATACCCTCGCTG | CAAATAGGACCTCTCTCTTATGGGAGCGAC |
| βA3Q85E | CTGTGGGCAAGAGTTTATCCTGG | GACACCCGTTCTCAAATAGGACC |
| βA3Q180E | CGTGGGTATGAGTATATCTTGG | GCACCCATACTCATATAGAACC |