Table 1 of Ji, Mol Vis 2010; 16:416-424. Table 1 of Ji, Mol Vis 2010; 16:416-424.
Table 1. Primers used to amplify DNA fragments encompassing the twelve microsatellite markers and the four single nucleaotide polymorphisms.
| Name | Primer sequence (5′-3′) | Length of product (bp) |
Annealing temperature |
|---|---|---|---|
| DXS8090 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTgggtgaaattccatcacaaa | 154–172 | 55 °C |
| R-acaaatgcagatgtacaaaaaata | |||
| rs11266282 | F-ccaaagatgaccgtgag | 666 | 60 °C |
| F-ctgccaatgttctggatgt | |||
| rs11771 | F-tggggttttaggtggtga | 350 | 56 °C |
| F-aaatgcaaagggtgatgc | |||
| DXS1069 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTagcctaacccacataacagc | 254–268 | 55 °C |
| R-agctactatattnaccttggtcttg | |||
| DXS1068 | F-cctctaaagcatagggtcca | 245–259 | 55 °C |
| R-cccatctgagaacacgctg | |||
| DXS8109 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTacaggctcggcttattaggg | 229–239 | 55 °C |
| R-5′-ctttcagtgccaggcatagg | |||
| rs6623918 | F-5′-tctatttccttactttcccaca | 436 | 58 °C |
| R-5′-ggaccctttccgcttgat | |||
| rs5923859 | F-5′-tattgttgtaaggtgggc | 379 | 56 °C |
| R-5′-cttggcttctgctgatat | |||
| DXS6803 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTgaaatgtgctttgacaggaa | 110–126 | 55 °C |
| R-5′-caaaaagggacatatgctactt-3′ | |||
| DXS1196 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTctaaattctcctccaccgtg | 209–227 | 55 °C |
| R-tttccagagcagattttcagt | |||
| DXS1222 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTgcaaaaatccccagcc | 234–240 | 55 °C |
| R-ttcattgccatccagattc | |||
| DXS8074 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTataaattagccagaggtgttg | 221–231 | 55 °C |
| R-5′-ctaggtgtgtctgtaaaggtagg-3′ | |||
| DXS1211 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTccctccaatctggcagaa | 159–175 | 55 °C |
| R-aagacctgggtttggcct | |||
| DXS984 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTtttctgtctgccaagtgttt | 154–184 | 55 °C |
| R-tactgngccctactccattc | |||
| DXS1205 | M13 tailed-F-CGTTGTAAAACGACGGCCAGTcctacgcatgtggctc | 184–202 | 55 °C |
| R-attaatggcttagagtactttttca | |||
| DXS1227 | F-agaggtccgagtcttccac | 77–99 | 55 °C |
| R-ataagggtttactcccccaa | |||
| M13 probe | CGTTGTAAAACGACGGCCAGT | 21 | x |