Figure 6. Extracellular signal-regulated kinase 1 and 2 (ERK1 and ERK2) mRNA levels in the neuroretina. ERK1 and ERK2 mRNA expression levels were assessed using real-time PCR in eyes subjected to ischemia and 5 (n=11), 12 (n=8), or 20 (n=6) h of reperfusion (I-R) in relation to sham-operated eyes. Note the initial reduction in both ERK1 and ERK2 after ischemia and 5 h of reperfusion. Values are presented as means±standard error of the mean. Statistical comparison was performed using a paired Student ratio t-test (ischemia versus sham-operated eyes) with Bonferroni correction.