Figure 5. Phosphorylated extracellular signal-regulated kinase 1 and 2 (ERK1/2) protein content in the neuroretina examined using western blot. Data are presented as (A) mean optical density and (B) representative western blots from animals subjected to ischemia and 5 (n=4), 12 (n=8), or 20 (n=5) h of reperfusion (I-R) and their corresponding sham-operated eyes. Note that phosphorylated ERK1/2 (p-ERK1/2) levels were higher in the eyes subjected to ischemia followed by 5 h of reperfusion. Values are presented as means±standard error of the mean. Statistical comparison was performed using a paired Student ratio t test (ischemia versus sham-operated eyes) with Bonferroni correction.