Figure 3. Glial fibrillary acidic protein mRNA levels in the neuroretina. Glial fibrillary acidic protein (GFAP) mRNA expression levels in retinas following ischemia and 5 h (n=6), 12 h (n=15), or 20 h (n=6) of reperfusion (I-R) were assessed by real-time PCR. The mRNA levels were calculated relative to the housekeeping gene β-actin (ACTB). The results are shown as mean values±standard error of the mean. Statistical analysis was performed using a paired Student ratio t test with Bonferroni correction for multiple comparisons. Note that the GFAP mRNA expression levels increase gradually with the duration of reperfusion.