Table 1 of Huang, Mol Vis 2010; 16:341-352. Table 1 of Huang, Mol Vis 2010; 16:341-352.
Table 1. Primers for RT–PCR.
Gene name |
Primers (5′–3′) |
Size of product (bp) |
Annealing temperature (°C) |
Cycles |
|---|---|---|---|---|
| Pax6 | F: 5′GACAGGGAGAAAACACCAACTC 3′ | 336 | 62.9 | 28 |
| R: 5′ TGGTACTGAAACTGCTGCTGAT 3′ | ||||
| Prox1 | F: 5′TGTTCTTTTACACCCGTTACCC 3′ | 363 | 62.1 | 35 |
| R: 5′CACTATCCAGCTTGCAGATGAC 3′ | ||||
| Cryaa | F: 5′GAGGGCTGAGGATTTGAGA 3′ | 268 | 53.1 | 26 |
| R: 5′ ATACGCCTGCGGTAAGTG 3′ | ||||
| Cryab | F:5′ GGCTAACCGACTCTACACTCA 3′ | 483 | 58.5 | 26 |
| R:5′ CTGTTTCCTTGGTCCATTCA 3′ | ||||
| Crybb1 | F: 5′ ACGATGGGACACCTGGAC 3′ | 206 | 57.9 | 26 |
| R: 5′ CACTGGAGACGGTTATACTGC 3′ | ||||
| Crybb2 | F: 5′ CCTCAGACCACCAGACACAG 3′ | 364 | 61.3 | 26 |
| R: 5′ CTCCATCTTCTTGCCCGTAA 3′ | ||||
| Cryba2 | F: 5′ CTCTGGGACGAGGAGGACTT 3′ | 352 | 61.7 | 26 |
| R: 5′ ATGGAAGGCAGTGATGGGTA 3′ | ||||
| Crygd | F: 5′ AGCACAGACCACTCCAACCT 3′ | 330 | 62.0 | 26 |
| R: 5′ AGCCCTCCAGCACATTGA 3′ | ||||
| G3pdh | F: 5′ACCACAGTCCATGCCATCAC 3′ | 439 | 60.7 | 24 |
| R: 5′TCCACCACCCTG TTGCTGTA 3′ |