Figure 5. Pax6 immunolocalization of rat lens regeneration from 3 days to 3 months after ECLE. Immunostaining for the Pax6 protein (green)
with nuclear counterstain DAPI (blue). Pax6 protein, normally found in epithelial cells and new lens fibers, is similar in
lens regeneration and normal lens development. The lens tissue is stained for Pax6. Note that the anterior capsule adheres
to the posterior capsule 3 days after ECLE (A, B); Pax6 is expressed in the lens epithelium and differentiating fibers on day 7(C, D); Pax6 is predominately located in the lens epithelium, and there is a low level of expression in the newly differentiated
lens fiber cells on day 14 (E, F; G, H);and finally, the level of Pax6 expression is reduced in the denucleated fibers 1 month after ECLE (in the lower right corner
of I, J). Abbreviations: e, lens epithelium; f, lens fiber cells; t, transition zone.