Figure 3. RT–PCR analysis for crystallin
genes of Cryaa, Cryab, Crybb1, Crybb2, Cryba2,
and
Crygd (encoding αA-, αB-, βB1-, βB2-, βA2-, and
γD-crystallin), and the transcription factors Pax6 and Prox1.
The
lens tissue is dissected at pre-operation, 0 h, 3 days, 7 days, 14
days, 1 month, and 3 months after ECLE and is then assayed. The size of
specific PCR products is shown in parentheses.