Molecular Vision: Hogewind, Mol Vis 2010; 16:26-35. Table 1

Table 1. WFS1 primers.

Exon	Forward primer (5′-3′)	Reverse primer (5′-3′)
2	TGTCTCCAGCAGACACTAAG	GGGTGGCTGAACCCCGTTC
3	GAAGACCCTCATGCCTTGTC	ATCTCAGGCACCGACACTTC
4	GGAGAATCTGGAGGCTGACT	ACAAGCTGCTCAACCCTCCA
5	AGAGTGGCACCGAAACCA	TCCTGTGGGAAGACCCAG
6	AACAGTGCGCCAGTTTCTG	GAGGCACGGGTGAGATAGG
7	CCCATTGCTCTGTGTGAGG	GAAGGTGCCCTGCCTGAG
8–1	AGAGGCAGGGTGGTCAGAG	GAGAGCAGGAAATGGGCATA
8–2	AGAACTTCCGCACCCTCAC	AGGTAGGGCACAAGGTAGCA
8–3	TATCTCTTCTTCCGCATGGC	TACTGCTGCCAGGTCAGTGT
8–4	CAAGCTCATCCTGGTGTGG	GTGACGTCGTCCTCCTCG
8–5	CCCTGCCACATCAAGAAGTT	GGTCTCTGCAGCCACAGTCT

The table shows the forward and reverse primers used for PCR amplification of the coding exons of the WFS1 gene. Exons 2 to 7 were amplified as single fragments, whereas the larger exon 8 was amplified in 5 overlapping fragments. PCR was performed using AmpliTaq Gold 360 Master mix (Applied Biosystems, Foster City, CA) with 50 ng genomic DNA and 10 pmol of each primer in a total volume of 25 µl. PCR reaction conditions for all fragments were: 95 °C for 10 min; then 35 cycles of 95 °C for 30s, 60 °C for 1 min and 72 °C for 30s, followed by 7 min at 72 °C.