Figure 5 of Dibas, Mol Vis 2010; 16:330-340.


Figure 5. Glial fibrillary acidic protein is upregulated following optic nerve crush, while B cell lymphoma-x (bcl-xl) is downregulated. Following retinal injuries with optic nerve crush, retinas were dissected and cytosolic proteins were isolated. Fifty micrograms protein was loaded into each lane. Glial fibrillary acidic protein (GFAP), a cellular marker for retinal injury, was upregulated (A: 450 ±190%, 262±30%, and 300±34%, days 2, 7, and 14, respectively, p<0.001, n=7). Bcl-xl (anti-apoptotic factor) downregulation is involved in initiating apoptosis and not surprisingly was reduced following optic nerve crush (B: 67±9% and 56±10%, at days 7 and 14, respectively, p<0.005, n=7). However, changes in bcl-xl protein levels at day 2 were not statistically significant.