Figure 1 of Chen, Mol Vis 2010; 16:310-319.


Figure 1. Effect of murine endostatin (mEndo), murine-soluble vascular endothelial growth factor receptor 2 (msFlk-1), and murine-soluble Tie2 (msTie2) expression on proliferation and migration of HUVEC cells. A: Equal numbers of HUVEC cells were incubated for 24 h in viral supernatant from vehicle vector-PT67, mEndo-PT67, msFlk-1-PT67, or msTie2-PT67 cells. The viable cell number was measured by the conversion during 4 h of MTT into soluble formazan. Bars represent standard deviation (SD; n=3 wells per measurement). Similar results were obtained in three independent experiments. B: HUVEC cells were seeded on 24-well tissue culture plates. When the cells reached 90% confluence, a wound was made with a micropipette tip in the center of the culture plates. The cultures were rinsed to remove detached cells and incubated with medium containing viral supernatant from vehicle vector-PT67, mEndo-PT67, msFlk-1-PT67, or msTie2-PT67 cells for 16 h. Digital images of wound closure were captured and used for quantitative assessment of migration by measuring the distance cells migrated beyond the injury lines. Four independent experiments were conducted, and data were shown as mean±SD. The asterisk indicates p<0.05, and the double asterisk indicates p<0.01, compared with vehicle vector cells.