Table 2 of Chiou, Mol Vis 2010; 16:294-302. Table 2 of Chiou, Mol Vis 2010; 16:294-302.
Table 2. Summary of identified phosphorylated proteins and phosphorylated sites in porcine lens proteins by IMAC followed by LC-MS/MS analysis.
| Protein [Accession number] |
Fragment |
Phosphopeptides |
Designation |
|---|---|---|---|
| αB-crystallin | 12–22 | RPFFPFHSPSR | Ser-19 |
| [Q7M2W6] | 12–22 | RPFFPFHSPSR | Ser-21 |
| 57–69 | APSWIDTGLSEMR | Ser-59 | |
| 57–69 | APSWIDTGLSEMR | Thr-63 | |
| 73–82 | DRFSVNLDVK | Ser-76 | |
| 124–149 | IPADVDPLTITSSLSSDGVLTVNGPR | Ser-139 | |
| 164–174 | EEKPAVTAAPK | Thr-170 | |
| αA-crystallin | 13–21 | ALGPFYPSR | Ser-20 |
| [P02475] | 55–65 | TVLDSGVSEVR | Ser-59 |
| 55–65 | TVLDSGVSEVR | Ser-62 | |
| 55–70 | TVLDSGVSEVRSDRDK | Ser-66 | |
| 79–88 | HFSPEDLTVK | Ser-81 | |
| 79–88 | HFSPEDLTVK | Thr-86 | |
| 146–157 | VPSGVDAGHSER | Ser-148 | |
| 146–157 | VPSGVDAGHSER | Ser-155 | |
| 158–173 | AIPVSREEKPSSAPTS | Ser-173 | |
| Beta-crystallin B1 | 8–21 | ASATAAVNPGPDGK | Ser-9 |
| [Q007T1] | 8–21 | ASATAAVNPGPDGK | Ser-11 |
| 88–107 | VRSIIVTSGPWVAFEQSNFR | Ser-90 | |
| 90–107 | SIIVTSGPWVAFEQSNFR | Ser-95 | |
| 185–199 | VSSGTWVGYQYPGYR | Thr-189 | |
| Beta-enolase | 81–89 | KLSVVDQEK | Ser-83 |
| [Q1KYT0] | 257–269 | YDLDFKSPDDPSR | Ser-263 |
| Heat shock protein beta-1 | 13–20 | SPSWDPFR | Ser-13 |
| [Q5S1U1] | 13–20 | SPSWDPFR | Ser-15 |
| Glucose-6-phosphate isomerase | 227–241 | EWFLQSAKDPSAVAK | Ser-232 |
| [NP_999495] | 448–461 | ELQAAGKSPEDFEK | Ser-455 |