Figure 5. Comparison of FLT-3 interacting zinc finger 1 (FIZ1) recruitment to gene promoter complexes during terminal maturation of photoreceptors. ChIP was performed using an antibody specific for the FIZ1 protein. Protein binding is proportional to the amount of target DNA captured by ChIP and is shown as copies of target DNA detected per 1,000 genome equivalents of input DNA. Results were normalized for chromatin input and PCR efficiency. Error bars indicate standard deviation (n=3). An untranslated region (Untr6) on chromosome 6 provided a negative-binding control. The 5′ end location of each PCR target is indicated with each gene name. For Example, Slc24a1-463 indicates that the qPCR target was 463 bases upstream of the Slc24a1 TSS. Rho, Rcvn, Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Grik2 and Casc5 were included as genes with ChIP-on-chip Pol-II peak signal ratios <1.0. Genes: Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1), Actin binding LIM protein1 (Ablim1), Recoverin (Rcvrn), Rhodopsin (Rho), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Retinoschisis 1 homolog (Rs1h), G protein-coupled receptor kinase 1 (Grk1), D4, zinc and double PHD fingers, family 3 (Dpf3), Retinol dehydrogenase-12 (Rdh12), Myeloid/lymphoid or mixed-lineage leukemia 6 (Mllt6), jumonji domain containing 2C (Jmjd2c), S-antigen (Sag), Phosphodiesterase 6b (Pde6b), Spectrin repeat containing, nuclear envelope 1 (Syne1), Cyclin-dependent kinase 5, regulatory subunit 2 (Cdk5r2), Vitronectin (Vtn), Cholecystokinin-2 receptor (Cckbr), Peripherin 2 (Rds), Uridine-cytidine kinase 1-like 1 (Uckl1), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Transducin alpha (Gnat1), Coiled coil domain containing 126 (6330407D12Rik, Ccdc126), Tetratricopeptide repeat domain 8 (Ttc8), Retinitis pigmentosa 1 (Rp1h), Retinol binding protein 3, interstitial (Rbp3), Coronin, actin binding protein, 2B (Coro2b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), G430022H21Rik, RAB14, member RAS oncogene family (Rab14), Elongation of very long chain fatty acids-like 4 (Elovl4), Ras homolog enriched in brain (Rheb), Histone deacetylase 10 (Hdac10), Histone deacetylase 9 (Hdac9), Ring finger protein 11 (Rnf11), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Cancer susceptibility candidate 5 (Casc5).