Figure 6. Characterization of healing of an alkali-burned cornea treated with systemic Trichostatin A (TSA) by real-time reverse transcription polymerase chain reaction (real-time RT–PCR) and immunohistochemistry. Real-time RT–PCR showed that systemic TSA treatment suppressed mRNA expression levels of a macrophage antigen, F4/80 (A), α-smooth muscle actin (αSMA; B), transforming growth factor β1 (TGFβ1; C) and collagen typeI (D) in a healing alkali-burned mouse cornea on day 5 and day 10. **p<0.01, *p<0.05. E: Immunohistochemistry also showed fewew myofibroblasts and macrophages in a TSA-treated healing alkali-burned mouse cornea compared with those in control mice. Bar, 100 µm.