Figure 1 of Kitano, Mol Vis 2010; 16:2964-2973.

Figure 1. Effect of Trichostatin A (TSA) on the expression of fibrogenic components in cultured human subconjunctival fibroblasts. A: Real-time RT–PCR showed that 10 nM of TSA suppressed mRNA expression of transforming growth factor β1 (TGFβ1) and collagen I a1 chain. B: TSA reduced the production of TGFβ1 at the protein level in the presence of exogenous TGFβ1. *p<0.05. C: Immunocytochemistry showed reduction of protein expression of collagen type I in the cell cytoplasm and deposition of fibronectin in the cell layer by adding concentrations of TSA. Bar, 100 µm. D: Effects of TSA on fibroblast migration were evaluated by scratch assay. A liner a defect was produced in a fibroblast monolayer. The cells migrated into the defect and the defect was closed in 36 h in the control culture. TSA retarded migration of fibroblasts into the defect. Bar, 200 µm.