Molecular Vision: Paliwal, Mol Vis 2010; 16:2955-2963. Table 1

Table 1. Table showing primer sequences and PCR conditions used for amplification of SLC4A11 and CHST6 genes.

SLC4A11 gene
	Primer Sequence 5′to 3′
Exons	Forward	Reverse	Annealing temperature (°C)
1	CCTAGCAGATGGGCTAAGCA	GAGCAAAGCCACAGGACTCT	60
2 and 3	CGAGAGTGGGACAGTCCAG	CTCCCTGTTGAGTGCTCCT	62
4 and 5	TCCAGGAGCAGCTCAACAG	CAGCCCTCTTCTCCCAAGTT	57
6	CCAACCAACTTGGGAGAAGA	CCTTCAGAGGCCAGGACAT	52
7 and 8	AAAACCTGCTGCCAGTTCAT	CCTAGGAATGGGGGATGG	57
9 and 10	ACTGATGGTACGTGGCCTCT	CGTCCATGCGTAGAAGGAGT	58
11 and 12	TCTACATCCAGGGTGCAGTG	CGTCCATGCGTAGAAGGAGT	56
13 and 14	GAGCCCTTTCTCCCTGAGAT	GGTTGTAGCGGAACTTGCTC	61
15 and 16	CGGGAAATCGAGAGTGAGTT	CGTCTCCTTCACGTTCACAA	54
17 and 18	CTGGCCACATGGGACATAG	CTAGGCAGGACCCCTCCTC	53.5
19	CAGGAGGGGCTCCAGTCTA	CTGTCCCTTGCATTCCACTT	55
Putative Promoter region 1	GCCTTACTCACCCAATCTATGC	CCCTGTCTCCTCCTTTCGAC	61
Putative Promoter region 2	GGAGGAGGAGAAGGACTTGC	GCACACTCGCGCACTCAC	55
CHST6 gene
	Primer Sequence 5′to 3′
Coding region	Forward	Reverse	Annealing temperature (°C)
1	GCCCCTAACCGCTGCGCTCTC-	GGCTTGCACACGGCCTCGCT	57
2	GACGTGTTTGATGCCTATCTGCCTTG-	CGGCGCGCACCAGGTCCA	55
3	CTCCCGGGAGCAGACAGCCAA	CTCCCGGGCCTAGCGCCT	57