Table 1 of Aparisi, Mol Vis 2010; 16:2948-2954. Table 1 of Aparisi, Mol Vis 2010; 16:2948-2954.
Table 1. Primers for USH1C gene.
| Exon | Primer | Sequence (5′-3′) | Size (bp) |
|---|---|---|---|
| 1 | 1D-N | CGACTCAGCACCTTCGACTC | 271 |
| 1R-N | TCCGGAGTCCCAGAAGCCTG | ||
| 2 | 2-D | GGTGGTCTGCATAGGTCTGA | 375 |
| 2-R | TCCAGGAGCCGTGAGCATC | ||
| 3–4 | 3–4-D | AGTGGTCTACTCCATTCCTAA | 625 |
| 3–4-R | CCGAAGGCTCAGAAAAGTGG | ||
| 5 | 5-D-N | TGCCACCTGAACCTGGGATC | 276 |
| 6-R | TAGAGCCTCCAGCCAGCCTCCAC | ||
| 5–8 | 5–8-D-N | GAGCATCGGTGGTGAGTCTG | 1191–1461
(the length of the amplicon is variable because of the presence of a VNTR in intron 5) |
| 5–8-R-N | TGAGGAAGGGGAGGGCAATAG | ||
| 9 | 9D | GGCTGAAGAGGTAGGCAGTC | 376 |
| 9R | AGGGTCAAACATCCCCAGTC | ||
| 10–12 | 10–12-D | CCACCAGAGCTTTCCAACTG | 937 |
| 10–12-R | ACAGCGGGCAGGAAGCAAG | ||
| 13–14 | 13–14-D | ATAACGTCCCCCAAAACCAA | 793 |
| 13–14-R | CACCAAGGGCTATCCATCTA | ||
| 15 | 15-D-N | ACCTCACAGCTCCCATGGAG | 285 |
| 15-R-N | CTGAAGCTGGGTGTCTGCAC | ||
| 16 | 16-D | TGTTCTGCAACCAAGGCAGG | 350 |
| 16-R | AACAGGCCAAGTCACACCATT | ||
| 17 | 17-D | GGCCTTCCTGTCCTAAACCTG | 441 |
| 17-R | GCTCACTCCACCCTTGTATGC | ||
| 18–19 | 18–19-D | CCTTGAGGGCCAGTTGGAACA | 1400 |
| 18–19-R | GAGGACATGGGAAACAGCAGT | ||
| 20 | 20-D | GCCGCTCAGTAGTTTCTGTG | 445 |
| 20-R | CTGCATTTTTGTCCCACCTC | ||
| 21 | 21-D-N | AGGGACATTGGCACGGCAG | 219 |
| 21-R-N | GAAGTGGCACAGAGTGGGAG | ||
| 22–24 | 22–24-D | CCATTCATCCCCCTACTCC | 1092 |
| 22–24-R | GTGGTCACCTGTTTGCTTTC | ||
| 25 | 25-D | TTTCAGAACCCAGGCTCAG | 316 |
| 25-R | GGCATCCTATTGTGAGACC | ||
| 26 | 26-D | TAGAAACGTCCTCAGACCAT | 339 |
| 26-R | GCTTGGGCCATTCCTTCAG | ||
| 27 | 27-D | GGAGCCCAGTGAAAGGAGAA | 295 |
| 27-R | GACGCCAGTCCAAAGAACCT | ||
| 28 | 28-D | TGCTCTGGCTGGGCTGAGT | 628 |
| 28-R | ATAGGGGCCACAAACCTTAT |