Figure 2 of Davidson, Mol Vis 2010; 16:2916-2922.

Figure 2. The ex vivo splicing assay. A: Schematic representation of the α-globin-fibronectin- extra domain B (EDB) splice assay construct. Wild-type and mutant (c.102C>T) forms of BEST1 exon 2 with flanking intronic sequence were cloned into the α-globin-fibronectin-EDB splice assay vector. The position of the mutated residue is highlighted with a star, and primer binding sites to exonic vector sequences are indicated with arrows. B: Splicing products generated by RT–PCR were separated by agarose gel electrophoresis as indicated. The identity of the spliced products was established by direct sequencing and is schematically represented on the right. C: Agarose gel of RT–PCR reactions performed with control primers designed against the vector sequence (dashed arrows in A) demonstrates equal loading of the cDNA template. The figure represents results obtained from three separate experiments.