Figure 2 of Dvoriantchikova, Mol Vis 2010; 16:2882-2890.

Figure 2. ATP-liposomes effectively pass through the blood–retinal barrier. A: Confocal photomicrographs of flatmounted ischemic retinas treated with carboxyfluorescein (CF)-liposomes or carrier buffer PBS were taken 24 h after ischemia reperfusion (IR). Nonfixed freshly isolated retinas were visualized by confocal microscopy in the ganglion cell layer (GCL). Retinal GCL cells display green fluorescence, indicating liposomal uptake (yellow arrows). Punctuate traces of CF labeling are likely endothelial cells in blood capillaries (red arrows). PBS-treated controls display little autofluorescence. The scale bar represents 50 µm. B: Relative changes in ATP (ATP) content in control (sham) and ischemic (24 h) retinas following the treatment with ATP, PC- liposomes, and PBS 24 h after reperfusion. The results are shown as a percentage of the corresponding value in PBS-treated sham-operated retinas (*p<0.05, n=5).