Figure 1. ATP-liposomes rescue retinal ganglion cell from necrosis after oxygen and glucose deprivation. A: Treatment by ATP (ATP)-liposomes results in neuroprotective effects in the retinal ganglion cell (RGC) primary cultures after 6 h of oxygen and glucose deprivation (OGD) following 12 h re-oxygenation compared to phosphatidylcholine (PC)-liposomes and PBS-treated RGCs; survival rate after OGD is expressed as a percentage of the mean value obtained in parallel cultures. B: Treatment with ATP-liposomes decreases necrotic cell death in the RGCs cultures following 6 h OGD and 12 h re-oxygenation. C: Necrotic and apoptotic cells were determined using Annexin V as a marker of apoptotic cells and Annexin V/propidium iodide (PI) to identify necrotic cells. The percentage of necrotic cells and apoptotic cells relative to the total number of cells was determined for each of ten images (*p<0.05, **p<0.01).