Figure 3. Still frames taken at the following time points in culture: A. Day 0, 3:45 h; B. Day 1, 11:15 h; C. Day 3, 17:03 h; D. Day 5, 14:03 h. These images highlight the basic findings of the time-lapse experiment, namely, the simultaneous enlargement of focal aggregates (proto-spheres) together with the progressive decrease in the overall number of aggregation centers. In the video this can be seen to largely correspond to the adherence of cells and cellular clusters over time.