Appendix 1 of Wang, Mol Vis 2010; 16:2850-2866.


Appendix 1: Time-lapse video record of cultured transduced retinal progenitor cells.

Appendix 1 sequence of genetically modified transduced retinal progenitor cells cultured with EGF+GDNF for 5 days. Data shown were captured in the incubator by the Incucyte Live Cell Monitoring System (Essen BioScience, Ann Arbor, MI). Attention to cell motion and cluster formation reveals that contact and adherence between dissociated cells plays a prominent role in the initial stages of sphere formation. The time counter is shown in the inset.