Table 2 of Lai, Mol Vis 2010; 16:2829-2838.
Table 2. Next-Generation sequencing default parameters run with and without application of Condensation Tool and best adjusted parameters.
Parameters | Default A | Default B | Best adjusted |
---|---|---|---|
Condensation tool | Yes | No | No |
Alignment | |||
Seed number | 30 | 30 | 35 |
Move step | 5 | 5 | 10 |
Matching base percentage | 80% | 80% | 85% |
Mutation filter (mutation percentage) | 20% | 20% | 25% |
Mutation score (optional) | Not applied | Not applied | Applied, score=5 |