Table 1 of Lai, Mol Vis 2010; 16:2829-2838.

Table 1. Primers used for sequencing of additional genes mapped to common PPCD1 interval in build 37.1.

Gene Exon Forward primer Tm (ºC) Reverse primer Tm (ºC) Product size (bp)
LOC100270804* 1A gcgtggtaatgtggctttgtacc 68.1 tcaacagtaaacgctgcacatcc 68.1 558
  1B actcgctccttcccgcaaatgta 71.4 ttcttttcagtcgacacatgcaa 66.6 592
  1C ctccctgacagacactggcctta 68.6 ggctacaaagagccccttcttga 68.4 552
  1D aagcggatgacctgtgttcactc 68.6 cggtcctgaggtagggctacagt 68.4 527
  1E agagggtcctgtcatccattgaa 67.8 ccgaactgtaccaaactcatgtgc 68.4 695
LOC100287054 1 gctgttgctgaccagggtgt 67.7 aggctcttctccctcccttgaat 68.2 299
  2 caaaaggacacagaggtgaactgg 67.8 ccatgaccaaccgatgctgt 68.3 486
  3 cacaacattgttccacggtctca 69.1 gcagacagggcagcctcaag 69.7 398
  4 ctggaggggagagggagagaag 68.6 agtagcgccgagaaatccgttac 68.1 395
LOC100287095 1 gcttgtgcctccagaccagaat 68.7 ccaccttggcctcccaaa 68.4 300
  2 ttaaaattgcccaaaacccaagg 68.0 tcacccacgtgcgatatttcttc 68.9 661
PET117 1 ctatgctcggctctcgattgct 68.7 accgcgggggaaagacac 69.6 384
  2 aactgggtatttggaatctgaaa 62.3 tgatcaagtttaaaaggacagtgacca 67.2 394

*The 2017 bp exon of LOC100270804 was amplified and sequenced as 5 overlapping segments.

Lai, Mol Vis 2010; 16:2829-2838. http://www.molvis.org/molvis/v16/a303

©2010 Molecular Vision http://www.molvis.org/molvis/

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