Figure 3. The mutant E107A protein
displays remarkably higher tendency to self-aggregate than wild type
HGDC. A: Relative emission maximum values of bis-ANS bound to
wild-type and E107A HGDC, as a function of probe concentration.
Excitation wavelength was 390 nm and emission 515 nm. B:
Similar comparison of the emission maximum of the probe Nile Red bound
to wild-type and E107A HGDC. Excitation maximum here was 540 nm and
emission monitored at 660 nm.