Figure 3. The mutant E107A protein displays remarkably higher tendency to self-aggregate than wild type HGDC. A: Relative emission maximum values of bis-ANS bound to wild-type and E107A HGDC, as a function of probe concentration. Excitation wavelength was 390 nm and emission 515 nm. B: Similar comparison of the emission maximum of the probe Nile Red bound to wild-type and E107A HGDC. Excitation maximum here was 540 nm and emission monitored at 660 nm.