Figure 1. The mutation E107A in HGDC does not significantly alter the protein conformation. A: Far-ultraviolet circular dichroism spectra of wild-type and E107A mutant HGDC. The protein concentrations used were 10 μM (0.2 mg/ml) in MOPS buffer, pH 7.3, cell path length 2 mm, and the spectra were recorded at room temperature. [Θ]_MRW refers to mean residue weight ellipticity, in degrees (MRW or mean residue weight taken as 110 Da). B: Near UV CD spectra of wild-type and E107A mutant HGDC. Protein concentrations and the other conditions of measurement were the same as in A, excepting that the cells used were of a 1 cm path length.