Table 1 of Kaul, Mol Vis 2010; 16:240-245. Table 1 of Kaul, Mol Vis 2010; 16:240-245.
Table 1. Primer sequences and annealing temperatures of GJE1.
GJE1 |
Forward primer |
Reverse primer |
Product size (bp) |
Annealing temperature (°C) |
|---|---|---|---|---|
| 5`UTR 1a | CATCATTCAGACCATACCACA | AACCCAGCAGAATTTACCAG | 350 | 54 |
| 5`UTR 1b | TGTCACCATGAGGGAGATAA | GCAAATTAGTGCTGATGCTG | 383 | 54 |
| 5`UTR 1c | TCACATCTAGCCCCATAACC | CCAAGACTCTGATTGCACCT | 466 | 55 |
| 5`UTR 1d | TCCACTCCAGCAGTACAGAA | GTTTTGGAGCAGAGGACAAG | 257 | 55 |
| Exon 1a | GGTGCAATCAGAGTCTTGGT | CCTTCCCCTTTCCTGATAAT | 491 | 54 |
| Exon 1b | TGAGCAGAGTGAATTCGTGT | AAGAGACAGAAACCGCTGAC | 474 | 54 |
| Exon 1c | TTGCAGTACCACCTGTATGG | TGCCTAGAACCTGGTTACCT | 452 | 55 |
| Exon 2 | GGTACGCACTGTGAAAAAGTT | GGTGCAAACATGGCTTTTAT | 315 | 55 |