Figure 3. ADAM/ADAMTS gene expression in response to mechanical trauma. Gene expression profiles were classified in CBs; at t=0 following in vitro sham cataract operation (left panel), at t=6d, following short-term culture (central panel) and ex vivo after cataract surgery (right panel). The cycle threshold number (C_T) was used to classify gene expression as either very high (C_T≤20), high (C_T=21–25), moderate (C_T=26–30), low (C_T=31–38), or negligible to undetected (C_T=39–40). Donor information; category (t=0, 6d, ex vivo), sex (F; female, M; male), age and ophthalmic history. t=0: F, 74, none; M, 83, none; M, 81, none. t=6d; M, 76, none; F, 76, none; M, 69, no info. Ex vivo; M, 75, bilateral cataract surgery 2001; F, 76, no info; M, 70, no info.