Figure 2. Lens crystallins expression in
murine corneas of various ages and strains. Positive immunoreactivity
is denoted by a brown color. A: Alpha-A expression in C57BL/6
corneas at different development ages. E16, embryonic day 16; P0, newly
born; w, weeks; m, months. Each sample was serially sectioned and the
ones near the corneal centers were used for immunohistochemistry.
Neighboring two sections were processed exactly same except for that
the primary antibody was omitted for one of them and used as staining
negative control (lower rows). Asterisks (*) in E16 and P0 samples
denote tissue of lens. B: Expression of lens crystallins in
murine corneal epithelium of C57BL/6 or Balb/c strains.