Figure 5. Immunofluorescence staining of mucin-5AC in the limbal epithelial cells treated with 100 ng/ml nerve growth factor. A few of goblet cells (green, white arrows) were detected at 24 h (A), the mumber of goblet cells increased at the time point of 5 d comparing with 24 h (D; p<0.05). Nuclei were stained with 4,6-diamidino-2-phenylindole (B, E; blue). Double immunolabeling for mucin-5AC and 4,6-diamidino-2-phenylindole indicated the presence of goblet cells in primary culture of mouse limbal epithelial cells with 100 ng/ml nerve growth factor (C, F).