Table 3 of Li, Mol Vis 2010; 16:231-239. Table 3 of Li, Mol Vis 2010; 16:231-239.
Table 3. Primers and restriction enzyme used in this study
SNP |
Primer sequence |
AT (°C) |
Enzyme |
PCR product |
Enzyme products |
|---|---|---|---|---|---|
| rs2509897 | F:TGGGAGCAGGTCTAGGATT | 60.5 | BslI (NEB) | 170 bp | 29 bp and 141 bp |
| R: CAGGAAGAGCTTTAGTGAG | |||||
| rs1005510 | F: GGCACAGTCCTCTAAAATAC | 57.7 | BbvI (NEB) | 204 bp | 102 bp and 102 bp |
| R: CCTGACTATCCCTCATCTT | |||||
| rs11603020 | F:TGGCAAAATGTGAGTCGTGTTCCT | 63 | BstNI (NEB) | 293 bp | 223 bp and 70 bp |
| R: GCCAGTTGGGATCCTCTGGGC | |||||
| rs2511989 | F: GAAGAAGGACTTTCAACTG | 54.8 | DdeI (NEB) | 102 bp | 21 bp and 81 bp |
| R:TGAGAGGCAAATTCACTC |