Figure 2. PCR restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing for all tag single nucleotide polymorphisms (SNPs). A: Restriction analysis for rs2509897 resulted in digestible fragment (C/C), undigestible fragment (T/T), and heterozygote (C/T). B: Direct sequencing confirmed the restriction patterns for rs2509897. C: Restriction analysis for rs1005510 resulted in digestible fragment (A/A), undigestible fragment (G/G), and heterozygote (A/G). D. Reverse sequencing confirmed the restriction patterns for rs1005510. E: Restriction analysis for rs11603020 resulted in digestible fragment (C/C), undigestible fragment (T/T), and heterozygote (C/T). F: Reverse sequencing confirmed the restriction patterns for rs11603020. G. Restriction analysis for rs2511989 resulted in digestible fragment (A/A), undigestible fragment (G/G), and heterozygote (A/G). H: Reverse sequencing confirmed the restriction patterns for rs2511989.