Table 1 of Regus-Leidig, Mol Vis 2010; 16:2690-2700.


Table 1. Primary antibodies used in light and electron microscopy

Antibody Antigen specificity Dilution/ species Source and catalog number Reference
Bassoon Amino acids 756–1,001 of rat Bassoon 1:2,500
Mouse, monoclonal Stressgen, Victoria, Canada; VAM-PS003E
Clone SAP7F407 [27,29,35]
Bassoon sap7f Amino acids 756–1,001 of rat Bassoon 1:16,000
Rabbit, polyclonal E. D. Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany [27,31,35]
Cacna1f (Pep3) Peptide sequence AEEGRAGHRPQLSELTN, located in the cytoplasmic loop between domains I and II of mouse Cacna1f 1:5,000
Rabbit, polyclonal Marion Maw, Otago University, New Zealand [20]
CtBP1 Amino Acids 345–441 of mouse CtBP1 1:5,000
Mouse, monoclonal BD Biosciences, Heidelberg, Germany; 612042 [27]
         
Kinesin II
KIF3A Against the 130 kDa heavy chain of sea urchin Kinesin 1:100
Mouse, monoclonal COVANCE/BabCO, Richmond, CA, USA; MMS-1 88P [27]
         
Piccolo 44a Amino Acids 2,172–2,361 of rat Piccolo 1:2,000
Guinea pig, polyclonal E. Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany [23,27,2931,35]
RIBEYE/ CtBP2 Amino Acids 361–445 of mouse RIBEYE B domain/CtBP2 1:10,000
Mouse, monoclonal BD Biosciences, Heidelberg, Germany; 612045 [23,27,29,31,35]
RIBEYE aa 101–207 of rat Ribeye A domain 1:1,000
Rabbit, polyclonal Synaptic Systems, Göttingen, Germany; 192103 [30]
         
RIM 1 Amino acids 596–705 of rat RIM1 containing part of PDZ domain 1:1,000
Rabbit, polyclonal Synaptic Systems, Göttingen, Germany; 140003 this study
RIM 2 Amino acids 925 – 1,114 of rat RIM 2 1:1,000
Rabbit, polyclonal Synaptic Systems, Göttingen, Germany; 140303 this study
Veli3 Synthetic peptide derived from the C-terminus of the rat Veli3 (MALS-3) protein 1:1,000
Rabbit, polyclonal Zymed, San Francisco, CA, USA; 51–5600 [28,29]