Figure 1. Enrichment of club-shaped and spherical-shaped ribbon material using EGTA treatment. A: The percentage of rod-shaped ribbons is significantly reduced in EGTA-treated retinas (EGTA) compared to ionophore A23187-treated or untreated retinas, while the percentage of club-shaped and spherical-shaped ribbon profiles is significantly increased (n=237 ribbon profiles for EGTA- and ionophore-treated retinas; n=294 ribbon profiles for untreated retinas; error bars=SD). B-G: Electron micrographs of rod (B, C, D) and cone (E, F, G) photoreceptor terminals, showing examples of different ribbon states (arrowheads): rod-shaped (B, E), club-shaped (C, F), and spherical-shaped (D, G). * p<0.05, ** p<0.01, *** p<0.001. Scale bars: 0.2 µm.