Figure 4. Induction of keratocyte markers in ADSC. A, B: ADSC were cultured in fibrin gels for 3 weeks in keratocyte differentiation medium. C, D: ADSC were cultured as pellet for 3 weeks in keratocyte differentiation medium. Immunofluorescent staining shows the presence of keratan sulfate with antibody J19 (green; A, C) or keratocan with antibody KeraC, (green; B, D). Red shows nuclear staining by propidium iodide. E: RT–PCR shows keratocan expression in (left to right) uncultured keratocytes (positive control), ADSC in fibrin gel, ADSC as pellet culture, ADSC in SCGM. Scale bars=20 μm.