Figure 2. Induction of adipocytes from ADSC. ADSC were induced to differentiate into adipocytes in ADM for two weeks as described in Methods. A, B: ADSC were fixed and stained with Oil Red O. C, D: ADSC without induction were stained with Oil Red O as control. E: mRNA for leptin was detected by RT–PCR. Leptin expression (Upper) and 18S (Lower). Lane 1, uncultured keratocytes; lane 2, ADSC; lane 3, ADSC in adipocyte induction medium. Scale bars: 100 μm (A, C); 50 μm (B, D).