Figure 1 of Du, Mol Vis 2010; 16:2680-2689.

Figure 1. Flow cytometric identification of side population from cultured human adipose-derived stem cells (ADSC). A: Passage-two ADSC stained with Hoechst 33342 were analyzed using 350-nm excitation with blue (635 nm) and red (488 nm) emission. Cells showing reduction of both blue and red fluorescence (side population cells) were analyzed as defined by the box outlined on the left. B: An analysis similar to (A) but with a preincubation in 50 μM verapamil before incubation with Hoechst 33342.