Figure 6. Retinal human transferrin (hTf) in rd10 mice intraperitoneally injected with hTf. A: Enzyme-linked immunosorbent assay (ELISA) was used to quantify hTf in lysat of neural retinas from rd10 mice daily injected intraperitoneally with 6, 12, 24, and 48 mg/ml of hTf solution from day 5 postnatally and sacrificed at 25 day postnatally. hTf concentration in rd10 mice injected was compared to hTf concentration in 3-week-old rd10/hTf and TghTf mouse retinas. Values were means±SEM, n=10. Mice injected with 12 mg/ml were compared with mice injected with 6 mg/ml. Mice injected with 24 mg/ml were compared with mice injected with 12 mg/ml. Mice injected with 48 mg/ml were compared with mice injected with 24 mg/ml. rd10/hTf mice were compared with TgTfH mice (*p<0.05; ** p<0.01; *** p<0.001; ns: not significant). B: hTf was immunolocalized on retinal sections of 3-week-old rd10 mice injected with 48 mg/ml of hTf. C: mouse Tf was immunolocalized on retinal sections of 3-week-old rd10 mice injected with 48 mg/ml of hTf. Abbreviations: Ch is choroid; GCL is ganglion cell layer; INL is inner nuclear layer; IS is inner segment; ONL is outer nuclear layer; OPL is outer plexiform layer; RPE is retinal pigmented epithelium; Rv is retinal vessel. Scale bars in B-C equal 100 μm.