Figure 4. Retinal degeneration in 3-week-old rd10 mouse was compared to 3-week-old rd10/wild type (WT) and rd10/human transferrin (hTf) mice. A-C: Central part of equatorial sections represent histology from 3-week-old rd10 (A), rd10/WT (B), and rd10/hTf (C) mouse retinas. Nuclei were stained with toluidin blue. D-E: Outer nuclear layer (ONL; D) and inner nuclear layer (INL; E) thickness were measured every 200 μm, along the entirety of retinal sections of rd10, rd10/WT, and rd10/hTf mice at 3 weeks postnatally. (black line) rd10 mice, (red line) rd10/hTf mice, (gray line) rd10/WT mice. Values represent means±SEM, n=6–8. rd10/hTf mice were compared to rd10 mice: * p<0.05; **p<0.001; ***p<0.0001; ns represents not significant. rd10/hTf mice were compared to rd10/WT mice (≠≠ p<0.001; ≠≠≠ p<0.0001; ns: not significant; ON is optic nerve). F-M: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was used to stain retinas from rd10 (F), rd10/WT (H), rd10/hTf (J), and TghTf (L) mice at 3 weeks postnatally. Nuclei were stained with 4’,6 Diamidino-2-phenyl-indole (DAPI): rd10 (G), rd10 WT (I), rd10/hTf (K), and TghTf (M). Abbreviations: GCL is ganglion cell layer; INL is inner nuclear layer; ONL is outer nuclear layer; RPE is retinal pigmented epithelium. Scale bars in A-C and F-M equal 100 μm.