Figure 3. Light responsiveness of PER2::LUC bioluminescence rhythm in RPE-choroid. A: The light pulse was given at Day 4 of PER2::LUC rhythms. B: The control pulse was given at Day 4 of PER2::LUC rhythms. Arrows indicate the times of the treatment. C: The double-plotted phase response curve to light stimuli in the PER2::LUC bioluminescence rhythm in the RPE-choroid. Black squares indicate control and white circles indicate the samples that received the light pulse. D: Data were divided into 4 bins in 6 h intervals for statistical analysis. No significant differences were observed between the control (black bars) and the experimental (white bars) groups (two-way ANOVA, p>0.1).