Figure 2. Inhibition of human retinal
pigment epithelial cell line (ARPE-19) cell proliferation by
glucosamine (GlcN), measured with a colorimetric test (WST-1) and cell
counting. A: ARPE-19 cells were cultured for 48 h at different
concentration of GlcN. The proliferation results are expressed as a
mean percentage of control proliferation. B: After 24 h
culture, the ARPE-19 cells were exposed to 2.5 mM or 5.0 mM GlcN for 3
days. During this time, the medium was not changed. The ARPE-19 cells
were exposed to 2.5 mM or 5.0 mM GlcN with either 10% fetal bovine
serum (FBS; C) or 10 ng/ml endothelial growth factor (EGF; D)
days (the medium with fresh GlcN included was changed
daily). The results are the means±SD of five independent experiments.
The difference in inhibition of ARPE-19 cell proliferation among
various concentrations of GlcN was compared with one-way ANOVA (ANOVA);
Tukey’s test was used for post-hoc analyses. * p<0.05, ** p<0.01,
and *** p<0.001 versus the control.