Figure 1. Viability of human retinal
pigment epithelial cell line (ARPE-19) cells and determination of the LC50
concentration in the presence of GlcN. ARPE-19 cells were cultured in
Dulbecco’s modified Eagle’s medium and 10% fetal bovine serum (FBS).
The medium was changed after 24 h, and glucosamine (GlcN) was added in
concentrations between 0 mM and 140 mM. After 24 h, viability was
determined with 2% trypan blue. The LC50 was 39.0 mM. An LC50
value is the concentration of a material that will kill 50% of the test